Clinical Lab Specimen

Blood Specimen Collection
24 Hour Urine Specimen
12 Hour Urine Specimen
2 Hour Urine Specimen
Clean Void Mid-Stream Urine Collection for Women
Clean Void Mid-Stream Urine Collection for Men
Semen Analysis (For Fertility Checks)


Fasting Specimens
If the physician has ordered a test to be performed on a"fasting" specimen, the patient should refrain from eating or drinking anything other than water for a minimum of 8 hours, with 12 hours being optimal prior to collection.

Blood Specimen Collection

Labeling
  1. Obtain blood specimens from patient through routine venipuncture techniques
  2. Ask all patients to identify themselves by spelling their last name
  3. All specimen tubes and other containers must have
    a) Patients full name (First and Last)
    b) A unique identifier ( Medical Record Number, Social Security Number, or birth date if no other identifier is available )
    c) Date and time of collection
    d) Initials of person collecting specimen

Tube Drawing Order

  1. When collecting multiple blood tubes the following is the recommended sequence:
    a) Blood Cultures
    b) Blue Top
    c) Red Top
    d) Gold SST Barrier
    e) Green Top, plain or SST
    f) Lavender Top
    g) Gray Top
  2. Mix ALL tubes with anticoagulants (especially Blue, Green, Lavender and Gray) by inverting 6-8 times after filling. Gold SST Barrier tubes and Red Top Tubes should be allowed to clot for at least 20 minutes before centrifuging.
  3. Do not remove the stopper on any blood tubes. Stoppers should remain on the tube prior to and during centrifugation. Barrier tubes must be centrifuged for at least 15 minutes at a speed of 3000-3500 RPM's. Centrifugation should occur within 30 minutes of drawing.
  4. When it is necessary to remove serum from a tube, use a disposable transfer pipette to place serum in a plastic screw top tube.
  5. Warning: All blood and body fluid should be considered as potentially infections. Glovers and a lab coat must be worn when procuring or handling blood and body fluid specimens. Blood procurement safety devices must be used when drawing blood. The safety devices and instruction on their use can be obtained from the McLaren Northern Michigan laboratory.

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24 Hour Urine Specimen

(NOTE: Any 24 hour period may be selected.)

Collection containers are available from the Northern Michigan Hospital Laboratory for 24 hour urine collections. Patients are provided with a collection container and asked to keep the specimen refrigerated or stored on ice during collection.

  1. Have the patient void at the start time of collection. Empty bladder. Discard this specimen and record start time.
  2. Collect all urine voided after start time for the next 24 hours.
  3. At 24 hours, the patient should void and add urine to the specimen container. Record stop time.
  4. Keep the urine cool (refrigerate or place on ice) during the collection.
  5. Return the container to the laboratory as soon as possible.
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12 Hour Urine Specimen

(NOTE: Any 12 hour period may be selected, but 7:00 p.m. - 7:00 a.m. is usually most convenient.)
  1. Have patient void at 7:00 p.m. to empty bladder. Discard this specimen. Record start time.
  2. Collect all urine voided after 7:00 p.m. - 7:00 a.m.
  3. Keep the container refrigerated or on ice during collection.
  4. Return the container to the laboratory as soon as possible
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2 Hour Urine Specimen

Urine quantitative amylase determinations are often made on 2 hour collections. It is possible to obtain results on random urines, but these results cannot be calculated in units / specimen and no reference ranges are available for these results. Any two hour period is acceptable.

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Clean Void Mid-Stream Urine Collection for Women

( Three towelettes or gauze sponge in cleansing solution are required.)
  1. Sit as far back as possible on the toilet.
  2. Separate the folds of skin around the urinary opening. Use the three separate towelettes to perform the following three steps:
    a) Wipe once down the center of the urinary opening, front to back, discard towelette.
    b) Wipe once down one side of separated skin fold, front to back, discard towelette.
    c) Wipe once down the other side of separated skin fold, front to back, discard towelette.
  3. Start to urinate into the toilet - while continuing to urinate, place cup under stream of urine and fill to 1/2 or 3/4 full.
  4. Finish urinating in toilet .
  5. Use lid to seal urine cup tightly.
  6. Return specimen to nurse.
  7. Refrigerate until transported / tested.
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Clean Void Mid-Stream Urine Collection for Men

( Three towelettes or gauze sponge in cleansing solution are required.)
  1. If uncircumcised, pull back the foreskin and cleanse the area beneath with the sterile wipes provided. If circumcised, cleanse the head of the penis.
  2. Begin to urinate, DO NOT COLLECT the first part of the stream.
  3. While continuing to urinate, place the sterile cup in the urine stream and fill it only 1/2 to 3/4 full.
  4. Finish urinating in the toilet.
  5. Use lid to seal urine cup tightly.
  6. Return specimen to nurse.
  7. Refrigerate until transported / tested.
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Semen Analysis (For Fertility Checks)

The Specimen should be collected by the patient at home. Specimen should be ejaculated directly into a clean dry wide mouth plastic container, provided by the physician. (Urine cup is preferred). Give the patient a Semen Analysis Request Form. Do Not Use a condom to collect. Transport to the laboratory within ONE HOUR after collection. The entire specimen should be submitted since the determination of the volume is an important portion of the test.

  1. Note the collection time on the container
  2. Keep specimen as close to body temperature as possible.

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