Microbiology Specimen Collection

Labeling
Transport Media

   Vacutainer Midstream Urine Collection Kit
   Sterile Screw Top Container
   Para-Pak EcoFix (green cap)
   Para-Pak C & S Transport (orange cap)
   Bactec Blood Culture Bottles
   Isolation Tube
   Copan Swab (pink cap with gel in base)
   BBL CultureSwab or Culturette (regular and mini-tip)
   Copan Stuart's Swab (red cap with foam sponge in base)
   Micro Test Swabs for GC and Chlamydia Testing
   Pinworm Paddle
   Charcoal Swab
   Influenza and RSV Nasopharyngeal Swab


Labeling

  • Obtain specimen from the patient.
  • All specimen tubes and containers must have:
    • The patients full name (first and last).
    • A unique identifier (medical record number, social security number, or date of birth if no other identifier is available.
    • Date and time of collection.
    • Initials of the person collecting the specimen.

Transport Media

Vacutainer Midstream Urine Collection Kit

  • Uses
    • Urine Cultures
  • Instructions
    • Collect clean catch midstream urine or a catheterized urine sample in a sterile container.
    • Place the cannula end of the urine transfer device into the urine.
    • Insert the grey-stopper vacutainer tube onto the adapter and allow the tube to fill.
  • Notes
    • The urine specimen in the vacutainer transport tube must be delivered to the laboratory with 24 hours (outpatients).
    • Send the unpreserved urine if a urinalysis, urine chemistry, or a drug screen is ordered.
Laboratory Transport Media

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Sterile Screw Top Container

  • Uses
    • Catheter tips
    • Large volume body fluids
    • Prosthetic hardware
    • Sputum specimens
    • Tissue specimens for culture
    • Urine for urinalysis, drug screens, or chemistries
    • Clostridium difficile antigen ( CDT)
    • Stool for WBC (fecal leukocytes)
  • Instructions
    • Place the specimen in the container and transport immediately.
  • Notes
    • Keep refrigerated if transport is delayed
Sterile Screw Top Container

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Para-Pak EcoFix (green cap)

  • Uses
    • Giardia/Cryptosporidium testing
    • Full parasite exams.
  • Instructions
    • Select a bloody, slimy, or watery area of the stool.
    • Use the collection spoon provided in the container cap to transfer stool from a collection container to the fill line in the holding medium.
    • Mix well by shaking
  • Notes
    • Do not overfill.
    • Avoid contaminating the specimen with urine.
    • The holding medium is a skin irritant. Wash splashes with copious amounts of water.
Para-Pak EcoFix (green cap)

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Para-Pak C & S Transport (orange cap)

  • Uses
    • Stool Culture
  • Instructions
    • Select a bloody, slimy, or watery area of the stool.
    • Use the collection spoon provided in the container cap to transfer the stool from a collection container to the fill line in the holding medium.
    • Mix well by shaking.
  • Notes
    • Stool specimens for culture in the Para-Pak C & S Transport must be delivered to the laboratory within three days of collection.
Para-Pak C & S Transport (orange cap)

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Bactec Blood Culture Bottles

  • Uses
    • Blood Cultures
  • Instructions
    • Routine Blood Culture, Adult
      • Draw 20 ml of blood.
      • Place 10 ml in the aerobic Bactec bottle.
      • Place 10 ml in the anaerobic Bactec bottle.
    • Routine Blood Culture, Adult -- Short Draw (less than 13 ml of blood)
      • 5 ml -- Add all to a Peds Bactec bottle or to an aerobic Bactec bottle.
      • 5-10 ml -- Add all to a Bactec aerobic bottle.
      • 11-12 ml -- Add 5 ml to an anaerobic bottle, and the remainder to an aerobic bottle.
    • Routine blood culture, Child (less than 16 years old)
      • Add 1-5 ml of blood to a Peds Plus/F bottle. If a Peds bottle is not available, substitute an aerobic Bactec bottle.
  • Notes
    • Disinfect stopper with alcohol before adding the specimen
Bactec Blood Culture Bottles

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Isolation Tube

  • Uses
    • Blood cultures for AFB (TB) or fungus.
  • Instructions
    • Disinfect stopper with 10% PVP iodine.
    • Add 10 ml of blood.
    • Mix well.
Isolation Tube

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Copan Swab (pink cap with gel in base)

  • Uses
    • Most routine bacterial and fungal cultures such as
      • Wound
      • Eye
      • Ear
      • Throat
      • Urogenital
      • Anaerobe
      • MRSA, (Methicillin Resistant Staphylococcus Aureaus)
      • Staphylococcus cultures
  • Notes
    • Do not use the Copan Swab for
      • Viral testing or Chlamydia cultures
      • Tissue specimens
      • Catheter tips
      • Trichomonas wet preps
Copan Swab (pink cap with gel in base)

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BBL CultureSwab or Culturette (regular and mini-tip)

  • Uses
    • Viral culture or DNA probe
    • Diphtheria culture
    • Nasal culture (use mini-tip)
    • Trichomonas wet prep (not the optimal specimen; see below)
    • Urethral culture (use mini-tip)
BBL CultureSwab or Culturette (regular and mini-tip)

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Copan Stuart's Swab (red cap with foam sponge in base)

  • Uses
    • Rapid Strep A antigen from throat
  • Notes
    • Transport to laboratory immediately.
Copan Stuart's Swab (red cap with foam sponge in base)

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Micro Test Swabs for GC and Chlamydia Testing

  • Uses
    • Chlamydia and GC antigen tests (Chlamydia and GC direct tests)
  • Notes
    • This specimen cannot be used for cultures.
    • This testing method is not currently approved for testing in cases of suspected sexual abuse.
male GC and chlamydia test swab
Male Collector
female GC and chlamydia test swab
Female Collector

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Pinworm Paddle

  • Uses
    • Pinworm testing
  • Instructions
    • Press the sticky side of the paddle against the skin around the rectum.
    • Place the paddle into the plastic tube and transport to the lab.
Pinworm Paddle

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Charcoal Swab

  • Uses
    • Bordetella Pertussis (whooping cough)
  • Instructions
    • Call the Microbiology department for the correct swab and directions for

Charcoal Swab

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Influenza and RSV Nasopharyngeal Swab

  • Uses
    • Influenza testing
    • RSV testing
  • Instructions
    • Collect a nasopharyngeal swab and transport to the laboratory immediately at room temperature or refrigerated.
    • AFFIRM specimen collection container for Trichomonas, Gardnerella, and Candida
Influenza and RSV Nasopharyngeal Swab
Influenza and RSV Nasopharyngeal Swab container

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