Microbiology Specimen Collection

Labeling
Transport Media

   Vacutainer Midstream Urine Collection Kit
   Sterile Screw Top Container
   Para-Pak EcoFix (green cap)
   Para-Pak C & S Transport (orange cap)
   Bactec Blood Culture Bottles
   Isolation Tube
   Copan Swab (pink cap with gel in base)
   BBL CultureSwab or Culturette (regular and mini-tip)
   Copan Stuart's Swab (red cap with foam sponge in base)
   Micro Test Swabs for GC and Chlamydia Testing
   Pinworm Paddle
   Charcoal Swab
   Influenza and RSV Nasopharyngeal Swab


Labeling

  • Obtain specimen from the patient.
  • All specimen tubes and containers must have:
    • The patients full name (first and last).
    • A unique identifier (medical record number, social security number, or date of birth if no other identifier is available.
    • Date and time of collection.
    • Initials of the person collecting the specimen.

Transport Media

Vacutainer Midstream Urine Collection Kit

  • Uses
    • Urine Cultures
  • Instructions
    • Collect clean catch midstream urine or a catheterized urine sample in a sterile container.
    • Place the cannula end of the urine transfer device into the urine.
    • Insert the grey-stopper vacutainer tube onto the adapter and allow the tube to fill.
  • Notes
    • The urine specimen in the vacutainer transport tube must be delivered to the laboratory with 24 hours (outpatients).
    • Send the unpreserved urine if a urinalysis, urine chemistry, or a drug screen is ordered.
Laboratory Transport Media

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Sterile Screw Top Container

  • Uses
    • Catheter tips
    • Large volume body fluids
    • Prosthetic hardware
    • Sputum specimens
    • Tissue specimens for culture
    • Urine for urinalysis, drug screens, or chemistries
    • Clostridium difficile antigen ( CDT)
    • Stool for WBC (fecal leukocytes)
  • Instructions
    • Place the specimen in the container and transport immediately.
  • Notes
    • Keep refrigerated if transport is delayed
Sterile Screw Top Container

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Para-Pak EcoFix (green cap)

  • Uses
    • Giardia/Cryptosporidium testing
    • Full parasite exams.
  • Instructions
    • Select a bloody, slimy, or watery area of the stool.
    • Use the collection spoon provided in the container cap to transfer stool from a collection container to the fill line in the holding medium.
    • Mix well by shaking
  • Notes
    • Do not overfill.
    • Avoid contaminating the specimen with urine.
    • The holding medium is a skin irritant. Wash splashes with copious amounts of water.
Para-Pak EcoFix (green cap)

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Para-Pak C & S Transport (orange cap)

  • Uses
    • Stool Culture
  • Instructions
    • Select a bloody, slimy, or watery area of the stool.
    • Use the collection spoon provided in the container cap to transfer the stool from a collection container to the fill line in the holding medium.
    • Mix well by shaking.
  • Notes
    • Stool specimens for culture in the Para-Pak C & S Transport must be delivered to the laboratory within three days of collection.
Para-Pak C & S Transport (orange cap)

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Bactec Blood Culture Bottles

  • Uses
    • Blood Cultures
  • Instructions
    • Routine Blood Culture, Adult
      • Draw 20 ml of blood.
      • Place 10 ml in the aerobic Bactec bottle.
      • Place 10 ml in the anaerobic Bactec bottle.
    • Routine Blood Culture, Adult -- Short Draw (less than 13 ml of blood)
      • 5 ml -- Add all to a Peds Bactec bottle or to an aerobic Bactec bottle.
      • 5-10 ml -- Add all to a Bactec aerobic bottle.
      • 11-12 ml -- Add 5 ml to an anaerobic bottle, and the remainder to an aerobic bottle.
    • Routine blood culture, Child (less than 16 years old)
      • Add 1-5 ml of blood to a Peds Plus/F bottle. If a Peds bottle is not available, substitute an aerobic Bactec bottle.
  • Notes
    • Disinfect stopper with alcohol before adding the specimen
Bactec Blood Culture Bottles

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Isolation Tube

  • Uses
    • Blood cultures for AFB (TB) or fungus.
  • Instructions
    • Disinfect stopper with 10% PVP iodine.
    • Add 10 ml of blood.
    • Mix well.
Isolation Tube

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Copan Swab (pink cap with gel in base)

  • Uses
    • Most routine bacterial and fungal cultures such as
      • Wound
      • Eye
      • Ear
      • Throat
      • Urogenital
      • Anaerobe
      • MRSA, (Methicillin Resistant Staphylococcus Aureaus)
      • Staphylococcus cultures
  • Notes
    • Do not use the Copan Swab for
      • Viral testing or Chlamydia cultures
      • Tissue specimens
      • Catheter tips
      • Trichomonas wet preps
Copan Swab (pink cap with gel in base)

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BBL CultureSwab or Culturette (regular and mini-tip)

  • Uses
    • Viral culture or DNA probe
    • Diphtheria culture
    • Nasal culture (use mini-tip)
    • Trichomonas wet prep (not the optimal specimen; see below)
    • Urethral culture (use mini-tip)
BBL CultureSwab or Culturette (regular and mini-tip)

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Copan Stuart's Swab (red cap with foam sponge in base)

  • Uses
    • Rapid Strep A antigen from throat
  • Notes
    • Transport to laboratory immediately.
Copan Stuart's Swab (red cap with foam sponge in base)

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Micro Test Swabs for GC and Chlamydia Testing

  • Uses
    • Chlamydia and GC antigen tests (Chlamydia and GC direct tests)
  • Notes
    • This specimen cannot be used for cultures.
    • This testing method is not currently approved for testing in cases of suspected sexual abuse.

Male Collector

Female Collector

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Pinworm Paddle

  • Uses
    • Pinworm testing
  • Instructions
    • Press the sticky side of the paddle against the skin around the rectum.
    • Place the paddle into the plastic tube and transport to the lab.
Pinworm Paddle

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Charcoal Swab

  • Uses
    • Bordetella Pertussis (whooping cough)
  • Instructions
    • Call the Microbiology department for the correct swab and directions for

Charcoal Swab

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Influenza and RSV Nasopharyngeal Swab

  • Uses
    • Influenza testing
    • RSV testing
  • Instructions
    • Collect a nasopharyngeal swab and transport to the laboratory immediately at room temperature or refrigerated.
    • AFFIRM specimen collection container for Trichomonas, Gardnerella, and Candida
Influenza and RSV Nasopharyngeal Swab
Influenza and RSV Nasopharyngeal Swab

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